Macrophage cell culture
Posted 08 September 2009 - 05:40 AM
Posted 08 September 2009 - 04:25 PM
Posted 14 September 2009 - 04:30 AM
cellular debris is my bet, small portions of the cell that have remained intact after apoptosis.
Okay, but why are so many of the cells apoptotic? (I will check them by FACS soon) I thought maybe the too high concentration of antibiotics/antifunglas was harmful, but then I found the cells even less healthy (but not contaminated!) when cultured without antibiotics/antifungals. They look much happier with antibiotics, apart from the high number of this "cell debris"...
And why are many of these "particles" apparently "attached" to the cells' surface? Or is not attachment but some kind of "budding" of the cells??
My medium consists of RPMI+Glutamine+FCS+Chicken serum+Triptose Phosphate Broth (+ P/S + AmphotericinB). I've always used this medium and did not have this problem before.
Thanks for your help!
Posted 14 September 2009 - 07:16 AM
I have seen these come off my FDCs; I think they are some sort of exosome. See if you can collect them and analyse the nucleic acid content.
Edited by lab rat, 14 September 2009 - 07:19 AM.
Posted 14 September 2009 - 05:18 PM
Posted 22 September 2009 - 05:58 AM