Hi, I intend to carry out a multiplex PCR protocol from a journal and they indicated the use of 0.01% gelatin and 6% sucrose. I would like to know what are these chemicals' purposes/functions in the PCR reaction. Thank you!
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Legendary Graduate Beggar
Posted 09 September 2009 - 10:27 PM
Hi,
I had done multiplex PCR before but never before I use anything that you mentioned. Can you explain further and maybe tell us about the journal you are referring to?
I had done multiplex PCR before but never before I use anything that you mentioned. Can you explain further and maybe tell us about the journal you are referring to?
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..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn’t kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn’t kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
