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Multiplex PCR


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#1 lysjoshua

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Posted 08 September 2009 - 01:24 AM

Hi, I intend to carry out a multiplex PCR protocol from a journal and they indicated the use of 0.01% gelatin and 6% sucrose. I would like to know what are these chemicals' purposes/functions in the PCR reaction. Thank you!

#2 Adrian K

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Posted 09 September 2009 - 10:27 PM

Hi,

I had done multiplex PCR before but never before I use anything that you mentioned. Can you explain further and maybe tell us about the journal you are referring to?
:huh:
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