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"Flies Attack" in Lab


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#1 Adrian K

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Posted 07 September 2009 - 08:07 PM

Dear all,

I am working in microbiology lab and we do prepare media in the same lab. However, we do face "flies attack" problem although we done everything we can to keep clean.

Is there any efficient way that we can keep flies out of our lab?

Thank you.

;)
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#2 vetticus3

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Posted 07 September 2009 - 09:13 PM

doors closed... windows closed... work in a hood or a designated room.

#3 LostintheLab

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Posted 07 September 2009 - 10:29 PM

View Postvetticus3, on Sep 8 2009, 02:13 PM, said:

doors closed... windows closed... work in a hood or a designated room.


Some sticky fly traps around the area you prepare the media if you can't work in the above conditions?
I"ve never had this problem, but maybe because it was too cold for flies or that we had no access to open windows.
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#4 gebirgsziege

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Posted 07 September 2009 - 10:29 PM

What sort of flies? And where do they come from? Do they come in from the outside or do they hatch out of your samples???
We are facing the problem of "small" flies (different species) or much worse mites from time to time when working with soil samples....so we keep soil etc. at a different place away from lab and media.
When the invasion comes from the outside see vetticus3s comment....
A man cannot be too careful in the choice of his enemies. (Oscar Wilde)

#5 Adrian K

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Posted 09 September 2009 - 06:44 PM

Hi All,

Yes, we work in the hood, and we do closed the doors but it doesn't help much. We don't have a special media room either, we use one room to do everything.

The small flies is similar with locally called fruit flies. It is around 3-5mm length. I'm not sure the scientific name, Drosophilla???

I really got no idea where they from. I don't think they hatch out from my samples because we discard our plates and empty our bins daily.

Any similar encounters?

:huh: :( :o
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn’t kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434

#6 vetticus3

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Posted 09 September 2009 - 09:11 PM

does anyone in your lab/building work with drosophilla?

does anyone regularly bring fruit for lunch?

what samples do you work with?

V

#7 Adrian K

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Posted 09 September 2009 - 10:24 PM

Hi, Vetticus

As far as I concern, nobody in our building, department or faculty is dealing with drosophillaresearch.

Fruits for lunch...i don't think so.

Perhaps the flies were from the mortuary below? :huh: I'm not sure.

I work with burkholderia pseudomallei.
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn’t kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434

#8 gebirgsziege

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Posted 10 September 2009 - 03:21 AM

Have you tried to clean up your lab from bottom to top and discharge or clean everything contaminated with the flies? They must have some place where they can propagate.
A man cannot be too careful in the choice of his enemies. (Oscar Wilde)

#9 phage434

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Posted 10 September 2009 - 03:59 AM

Leave out dishes with 1/2 vol cider vinegar and 1/2 vol dish washing soap to trap fruit flies.

#10 hobglobin

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Posted 10 September 2009 - 04:09 AM

First he or she should determine what it is, i.e. Drosophilas or Calliphoridae/Muscidae (if they come from the mortuary) or any other family and where they come from (windows, doors or a site inside the lab). Then one can start to control them with traps or the other measures. If they have e.g. Muscidae and then use Drosophila traps that attract them then they will have finally both....

Edited by hobglobin, 10 September 2009 - 04:10 AM.


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...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

#11 Adrian K

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Posted 10 September 2009 - 04:11 AM

Hi all,

Yes, I had clean the lab from bottom up...I was wondering whether flies can hatch inside our laminar hood or incubator, i mean the internal machinery part.???

Dear phage434,
do I need to shake the "vinegar trap" to create bubbles in order to catch flies?

Anyway, I will try the cider vinegar+washing soap. Meanwhile if I can catch a fly sample I will keep it posted here.

Have to go shopping using private money to buy vinegar...haha.
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn’t kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434

#12 hobglobin

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Posted 10 September 2009 - 04:21 AM

View Postadrian kohsf, on Sep 10 2009, 02:11 PM, said:

Hi all,

Yes, I had clean the lab from bottom up...I was wondering whether flies can hatch inside our laminar hood or incubator, i mean the internal machinery part.???


If there are rotting fruits (for Drosos) or dead animal(s) for the scavengers then it's possible.

One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

#13 pito

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Posted 11 September 2009 - 04:45 AM

Haha, funny topic.

I had some problems myself latetly with flies.
I had them in my agar etc... everytime I made a bottle and autoclaved it, there would be a fly in it haha.
Oh well, the fly is sterilised too and in the laminair flow, they get sucked away each time.

I think the fruit thingie is indeed a good idea: if they eat some fruit and trow it in the garbage , you can bet your life on it that you will get flies.

Or I just attrack flies ? Maybe you too? haha

I would hang some flystrips and like phage343 said, try out dishes with 1/2 vol cider vinegar and 1/2 vol dish washing soap to trap fruit flies or try it with some sugarwater with some soap in it, that works too.
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#14 lab rat

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Posted 11 September 2009 - 09:48 AM

Do you have a floor drain in the lab? Maybe it is under the safety shower. Sometimes flies will grow in the trap. (It happens with our facility, which has adjacent animal rooms.) Pour a disinfectant down the drain 1-2 times a week to get rid of them.
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#15 fluffybunny

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Posted 08 October 2009 - 11:17 PM

this topic definitely caught my attention. anyone out there ever experienced drosophilla attacking the lab when someone uses organic solvent?

everytime i run my column using chloroform, there's definitely a drosophilla hanging around at my bench. only one though, not a whole swarm.





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