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What about Colcemid and BrdU?

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#1 gardasil



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Posted 07 September 2009 - 06:58 PM

I'm trying to do cell harvesting here, so before that, I need to arrest the AF cells in metaphase. I came across some protocols using Colcemid solely, and sometimes using the combination of Colcemid and BrdU [5-bromo-2-deoxyuridine].

Anyone has experience in this, please share some of your methods.

Thank you. ;)

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