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Mutation during transformation/maxiprep


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#1 arnabde2000

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Posted 07 September 2009 - 04:39 PM

I have some DNA stock, a HA-protein in the lab left behind by some seniors. I transformed it and then maxipreped it. Now I see two minor mutations in the protein. Should I ask to repeat the sequencing, is a mistake in sequencing common (the peaks in the chromatogram clearly clearly shows the nucleotide mutation though). Should I pick up another colony and maxi-prep from that? My understanding is that theoritically all the colonies should have exactly the same DNA sequence (as it was made from a DNA stock). Please help.

Edited by arnabde2000, 07 September 2009 - 04:42 PM.


#2 vetticus3

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Posted 07 September 2009 - 09:16 PM

pick another colony
do a miniprep
sequence
if it is correct then do a maxiprep

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