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Help plz~ white mass in the mouse abdomen days after i.p. of tamoxifen


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#1 lydialing

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Posted 07 September 2009 - 02:44 AM

I did intraperitoneal injection of tamoxifen to mice due to experiment needs. At begining the mice looked normal but gradually became feeble and even died 3 days later. When open the abdomen, there's a lot of white mass amnog internal organs. The chemical is dissolved in corn oil and stored at -20 C degree, and thawed before use. The solution is clear after thawing and no particles can be seen. The dosage delivered is 10mg/mouse, (body weight ~24gram on the day of injection), which should be less than LD50 of tamoxifen.
Has anybody encounterd a problem like this? What may go wrong? Help needed, thank you very much.



#2 bob1

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Posted 07 September 2009 - 04:11 PM

Sounds like an inflammatory immune response, or that you punctured the gut!

#3 vetticus3

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Posted 07 September 2009 - 09:15 PM

my vote goes for punctured gut.




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