I just have a couple of questions regarding storage of some PCR components.
1) Is it better to dilute and store dNTPs in just water or PCR buffer 1X ?
I would prefer PCR buffer because water can be slightly acidic and also carbondioxide from air may dissolve in dNTP solutions causing PH to decrease further . Low PH causes dNTP hydrolysis as far as I know, so I'd think storing dNTPs in a buffered solution would be the best Practise . But yet I found too many using just water . So I'd like to share thoughts about that . What do you think is best ?
2) How many reactions should a single aliquot of dNTPs \ Primers be sufficient for ? How many freeze\thaw cycles do you think dNTPs \ Primers can withstand before problems can occur ?
3) I heard that Taq could be stable at 2-8 C for about 2 weeks ? is that true ?
Thanks for your time.
Edited by Bassaml7, 05 September 2009 - 07:40 AM.