I used MitoTracker Red (CMXRos) to detect variations in mitochondrial membrane potential (MMP) in a cell line treated with a drug in comparison with a control.
I want to quantify the loss/gain of MMP. What I did was to establish two regions in the control sample histogram, one to the left and one to the right of the maximum peak of fluorescence, and then I copied these regions to the drug treated samples. Is this a correct procedure? If not, where should I establish the regions?
Also, I was expecting loss of MMP. However, in two of the samples I get gain of MMP. What's the meaning of MMP gain?
Thanks for any reply.
Edited by cardosopedro, 05 September 2009 - 03:38 AM.