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Overxepression of Tagged construct for co IP


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#1 BioBus

BioBus

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Posted 04 September 2009 - 07:26 PM

When perform CoIP in C.elegans animals, I/ we detect most the endogenous level.

Beginning , now with mammalian cell line, if making stable cell line with a
bait-construct / prey ( any suggestion?) and then do IP. However, not sure
whether endogenous level of expression would be sensitive enough for Co-IP.

However, for quantitative analysis of protein interactions, bait:prey =1:1
molar ratio has been recommended.

So , any comment is greatly appreciated!
Bus !




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