Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

What's the principle for detecting the RNA degradation @ 300nm?

  • Please log in to reply
No replies to this topic

#1 hei2zhu



  • Active Members
  • Pip
  • 5 posts

Posted 04 September 2009 - 11:50 AM

In the instruction of RNase of USB. It says so.
"Assay Conditions:
Two units of RNase A are incubated with Torula yeast RNA in 0.05M sodium acetate, pH 5.0, in a volume of 3 ml at 25C. Absorbance at 300 nm is read at 15 second intervals for 4 min; incubation then continues at 25C. Absorbance is taken at 300 nm again after 3.5 hrs or until a steady state is obtained."

Can someone tell me why?

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.