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What's the principle for detecting the RNA degradation @ 300nm?


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#1 hei2zhu

hei2zhu

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Posted 04 September 2009 - 11:50 AM

In the instruction of RNase of USB. It says so.
"Assay Conditions:
Two units of RNase A are incubated with Torula yeast RNA in 0.05M sodium acetate, pH 5.0, in a volume of 3 ml at 25C. Absorbance at 300 nm is read at 15 second intervals for 4 min; incubation then continues at 25C. Absorbance is taken at 300 nm again after 3.5 hrs or until a steady state is obtained."

Can someone tell me why?




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