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Overblocking Western?


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5 replies to this topic

#1 bc87

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Posted 04 September 2009 - 03:19 AM

Because I was such an idiot and totally forgot I was doing western, I left the western at room temperature blocking overnight in casein. Is it still good? Or should I throw it out? ;)

#2 Dr Teeth

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Posted 04 September 2009 - 05:04 AM

Because I was such an idiot and totally forgot I was doing western, I left the western at room temperature blocking overnight in casein. Is it still good? Or should I throw it out? ;)


It will be fine as long as it didn't dry out. You can't overblock a blot.

Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley

#3 bc87

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Posted 04 September 2009 - 06:00 AM

Because I was such an idiot and totally forgot I was doing western, I left the western at room temperature blocking overnight in casein. Is it still good? Or should I throw it out? ;)


It will be fine as long as it didn't dry out. You can't overblock a blot.



Overnight in room temperature is alright?

#4 Dr Teeth

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Posted 04 September 2009 - 06:13 AM

Because I was such an idiot and totally forgot I was doing western, I left the western at room temperature blocking overnight in casein. Is it still good? Or should I throw it out? ;)


It will be fine as long as it didn't dry out. You can't overblock a blot.



Overnight in room temperature is alright?


Well, I wouldn't do it on purpose...but it should be alright if the blot didn't dry out.

Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley

#5 bob1

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Posted 06 September 2009 - 06:49 PM

Also so long as it didn't grow any bacteria.

#6 dxm

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Posted 07 September 2009 - 05:56 PM

You definitely do not need to throw it away. I never blocking overnight under RT, but I did do it for half day or so and it was fine. Just as Bob1 said, as long as no bacteria grows you are safe.

If you still worry about it, I think you can do a very short stripping and re-block it.




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