Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Alkaline phosphatase


  • Please log in to reply
5 replies to this topic

#1 Wolfie

Wolfie

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 03 September 2009 - 10:41 PM

HI,

My lab used to use Shrimp Alkaline phosphatase from Roche but the product has since been discontinued. I have found another althernative: Calf Intestinal Alkaline Phosphatase from NEB. I would like to ask if is there any difference using the one from calf intestine, in terms of efficiency and methodology?

Thank you....

#2 elpollodiablo

elpollodiablo

    member

  • Active Members
  • Pip
  • 22 posts
0
Neutral

Posted 04 September 2009 - 12:03 AM

HI,

My lab used to use Shrimp Alkaline phosphatase from Roche but the product has since been discontinued. I have found another althernative: Calf Intestinal Alkaline Phosphatase from NEB. I would like to ask if is there any difference using the one from calf intestine, in terms of efficiency and methodology?

Thank you....


Hi,

The main difference is that CIAP cannot be heat-inactivated as SAP can, so it is a bit more cumbersome to work with. I'd recommend that you find another supplier of SAP. I'm sure someone will be selling it.

Best regards.

#3 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,506 posts
253
Excellent

Posted 04 September 2009 - 04:51 AM

Also, check Antarctic Phosphase, by NEB, which can be heat killed.

#4 Jaaar

Jaaar

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 01 March 2012 - 10:19 PM

CIAP could be heat inactivated according to the product sheet of CIAP from Roche

#5 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,831 posts
415
Excellent

Posted 02 March 2012 - 12:36 PM

CIAP could be heat inactivated according to the product sheet of CIAP from Roche

They lie... it can be partially inactivated by prolonged incubation, but in general, it is pretty hard to inactivate.

#6 Jaaar

Jaaar

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 07 March 2012 - 01:23 AM


CIAP could be heat inactivated according to the product sheet of CIAP from Roche

They lie... it can be partially inactivated by prolonged incubation, but in general, it is pretty hard to inactivate.



I ran out of SEAP control enzyme for my Reporter Genn assay. Unfortunately the SEAP component is not sold separately and the recommended replacement from sigma is back-ordered...planning to use CIAP as a control and....Wish me luckPosted Image




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.