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resolving a 100bp difference at around 1.2kb (AGE)


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#1 leelee

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Excellent

Posted 03 September 2009 - 07:28 PM

Hi all,

I have deleted 100bp from a gene and want to check my clones to see which is right. I was wondering what the best AGE conditions are for differentiating between an approx 1.2kb PCR product band vs a 1.1kb band (ie what % gel would you use etc)......

Thanks.

#2 phage434

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Posted 04 September 2009 - 04:52 AM

This should be very obvious on a 1.2% agarose gel.

#3 leelee

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Posted 04 September 2009 - 08:19 PM

Thanks phage




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