Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

reconstitution of primers


  • Please log in to reply
2 replies to this topic

#1 chat25

chat25

    member

  • Active Members
  • Pip
  • 10 posts
0
Neutral

Posted 03 September 2009 - 08:59 AM

Hi all,

I need to know how to reconstitute a primer. actually i'm a fresher to this feild and this is the first time i'm running a PCR. the primer we bought has given the details as,

forward - Tm 64 oc 82.9 nmol
reverse - Tm 65.9 oc 63.4 nmol

i want to know how to use this primer to perform Gradient PCR.

Please help me out on this...

thanks

#2 fishdoc

fishdoc

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 272 posts
2
Neutral

Posted 03 September 2009 - 10:07 AM

Hi all,

I need to know how to reconstitute a primer. actually i'm a fresher to this feild and this is the first time i'm running a PCR. the primer we bought has given the details as,

forward - Tm 64 oc 82.9 nmol
reverse - Tm 65.9 oc 63.4 nmol

i want to know how to use this primer to perform Gradient PCR.

Please help me out on this...

thanks


Resuspend in water or tris to a final concentration of 100 uM. Use this as a stock solution and make a working solution of 10 uM, then add 2.5 ul of that to a 50 ul PCR reaction to get a final concentration of 0.5 uM per primer, or adjust to whatever concentration you want.

#3 chat25

chat25

    member

  • Active Members
  • Pip
  • 10 posts
0
Neutral

Posted 03 September 2009 - 07:59 PM

Hi all,

I need to know how to reconstitute a primer. actually i'm a fresher to this feild and this is the first time i'm running a PCR. the primer we bought has given the details as,

forward - Tm 64 oc 82.9 nmol
reverse - Tm 65.9 oc 63.4 nmol

i want to know how to use this primer to perform Gradient PCR.

Please help me out on this...

thanks


Resuspend in water or tris to a final concentration of 100 uM. Use this as a stock solution and make a working solution of 10 uM, then add 2.5 ul of that to a 50 ul PCR reaction to get a final concentration of 0.5 uM per primer, or adjust to whatever concentration you want.



thanks alot......




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.