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how to prepare RNAse A from powdered form


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#1 hazrina

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Posted 02 September 2009 - 11:51 PM

how to prepare RNAse A from powdered form for use in DNA extraction purification

#2 molgen

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Posted 03 September 2009 - 01:42 AM

Try this:

Dissolve RNAse A at a concentration of 10 mg/ml in 0.01M sodium acetate (pH 5.2);
Heat to 100C for 15 minutes;
Allow to cool slowly to room temperature;
Adjust the pH by adding 0.1 volumes of 1M TrisHCl (pH 7.4);
Dispense into aliquots and store at -20C.




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