Posted 02 September 2009 - 06:57 PM
I need to remove the EDTA from my DNasing step
will phenol purification also work?
Posted 03 September 2009 - 12:24 AM
1 volume of 7,5 M lithium chloride
1 volume of DNAse treated RNA sample
1 volume of nuclease free water
(so final concentration of lithium chloride will be 2,5 molar)
mix them, put -20 for at least 1 hour, overnight is always better, centrifuge at 15,300 rpm (or max speed on microcentrifuge) for 15-20 min at +4 C, discard the supernetant, add 1 ml of 70% EtoH (it should be -20 chilled), centrifuge at max speed for 15-20 min., discard the supernatant, dry the pellet, resuspend with nuclease free water.
it seems a bit long but to be in safe side this is the best way
Edited by microRNA, 03 September 2009 - 12:26 AM.
Posted 03 September 2009 - 12:25 AM
Edited by microRNA, 03 September 2009 - 12:27 AM.
Posted 03 September 2009 - 01:05 PM