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How long do you warm up media?


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9 replies to this topic

#1 iamdhan

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Posted 02 September 2009 - 05:54 PM

Any damage could be done to media if I warm it up too long?

Say....like half of a day before use?

I just forgot that I put it in 37'C water bath in the morning..

It was a one of those crazy days that i was mentally off..

Then 6 o'clock in the evening, same day, I just found a bottle of media, and was like "Hmm...that one seems familiar.........uh?......argg! it is mine!!!"

Would it be okay after extra hours of warming?

If not, why??

thx. :P

#2 Stephan

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Posted 02 September 2009 - 10:57 PM

if it is plain media I would go with it.

#3 madrius1

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Posted 03 September 2009 - 09:54 AM

I did forget one bottle over night at 37C. It was perfectly fine.

#4 iamdhan

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Posted 03 September 2009 - 06:07 PM

Cool. <_< silly me, i worry too much.

#5 pito

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Posted 04 September 2009 - 01:56 AM

Any damage could be done to media if I warm it up too long?

Say....like half of a day before use?

I just forgot that I put it in 37'C water bath in the morning..

It was a one of those crazy days that i was mentally off..

Then 6 o'clock in the evening, same day, I just found a bottle of media, and was like "Hmm...that one seems familiar.........uh?......argg! it is mine!!!"

Would it be okay after extra hours of warming?

If not, why??

thx. ;)


what media do you mean and for what? And was whatever you wanted to put in the media allready in it?

when I grow cells I put the media at 37C the evening before I use it, and sometimes even 1 day before that.
The media wont go bad... I so no reason why to worry.
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.

#6 bob1

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Posted 06 September 2009 - 05:16 PM

Cell culture medium containing L-glutamine should only be warmed for short periods of time as the L-glut is unstable. Long periods in the waterbath will cause it to degrade.

#7 toprak

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Posted 08 December 2009 - 02:43 PM

i never warm up the media in a water bath. I just take it out in the morning and bring it up to room temperature. and I never had any problems with both established lines and primary cells.

#8 sanjiun

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Posted 09 December 2009 - 01:14 AM

i never warm up the media in a water bath. I just take it out in the morning and bring it up to room temperature. and I never had any problems with both established lines and primary cells.


Ya. I would do the same if don't need the media immediately.
Otherwise I'll warm it in waterbath for 30 min if I am in hurry.

#9 lab rat

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Posted 09 December 2009 - 08:38 AM

Ya. I would do the same if don't need the media immediately.
Otherwise I'll warm it in waterbath for 30 min if I am in hurry.


I warm mine up in the waterbath for 30 min, too. I start the hood, put the medium in the waterbath, and have a cup of coffee. Then I'm ready to go. :) However, long periods of incubation at 37 C will also cause amphotercin to degrade as well as the L-glut.

Edited by lab rat, 09 December 2009 - 08:39 AM.

42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.

#10 bob1

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Posted 09 December 2009 - 03:37 PM

Ya. I would do the same if don't need the media immediately.
Otherwise I'll warm it in waterbath for 30 min if I am in hurry.


I warm mine up in the waterbath for 30 min, too. I start the hood, put the medium in the waterbath, and have a cup of coffee. Then I'm ready to go. :P However, long periods of incubation at 37 C will also cause amphotercin to degrade as well as the L-glut.

Amphoterecin???? you don't want that in your culture, antibiotics and especially antimycotics are bad - they can really screw up your culture and mask low level infections which will cause spurious results.




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