Dear All,
I want to detect the small protein with 14 kDa of mass. I had found on the Fermentas website that it is better to use the Acy/Bis Solution with C=5%. So, I went on the Bio-rad site but the readymade solutions of Acrylamide Bis are numerous and I am kinda confused which to use. So, if you have any experience with that then please let me know.
Thanks a lot in advance.
Niraj
0
3 replies to this topic
#2
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an elder
Posted 06 September 2009 - 06:55 PM
you should try the tris-tricine (shagger and von jagow) formulation for separating small proteins and peptides.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
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#3
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Enthusiast
Posted 08 September 2009 - 05:50 PM
mdfenko, on Sep 6 2009, 06:55 PM, said:
you should try the tris-tricine (shagger and von jagow) formulation for separating small proteins and peptides.
Thanks a lot buddy.
#4
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an elder
Posted 11 September 2009 - 08:36 AM
the formulation instructions for the tris-tricine gel gives the acrylamide/bis ratio (49.5%T, 3%C). i think the formulation is somewhere in this forum (i may have put it here). when i gave my initial response i was away from the lab and did not have access to my methods book.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
