Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Plyse cell and DH5a

  • Please log in to reply
1 reply to this topic

#1 shimshady



  • Active Members
  • PipPipPipPipPip
  • 37 posts

Posted 01 September 2009 - 12:23 PM

I have a question, im following the protocol from a paper and the plasmid they have is expressed in bl21 plyse cell line. My question is is i need to clone the plasmid, is it ok to do this in DH5a cell, or do i need a special kind? Thanks

#2 swanny



  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 368 posts

Posted 01 September 2009 - 08:22 PM

If you're just doing manipulations of the DNA, you can just put the plasmid through DH5. The pLysE plasmid in BL21 is to stop (hopefully) leaky protein expression, typically of toxic proteins. The expression plasmid should have a T7 promoter, so you won't get any expression in DH5, which lacks the gene for T7 RNA polymerase.
Heart disease kills more women than breast cancer, but heart attack symptoms differ from men's symptoms. Get to know your heart... it could save your life.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.