I'm trying to isolate nuclei from HeLa cells for nuclear run-off assays. Using my current protocol, the nuclei would lyse (becomes extremely viscous) when I add the transcription buffer for the assay. Does anyone have a successful protocol or tips?
0
1 reply to this topic
#1
softie
-
- Members
-
- 2 posts
member
0
Neutral
Neutral
Posted 16 April 2002 - 10:22 AM
Hi...
#2
ranjitkgiri
-
- Members
-
- 1 posts
member
0
Neutral
Neutral
Posted 02 August 2002 - 01:30 PM
Add RNase Free DNAaseI. That will take care of your viscous DNA and you will get a decent radilabelled RNA.
Ranjit K. Giri.
ranjitgiri@hotmail.com
