Hi,
Does anyoone know if the anti-FLAG antibody M2 cross reacts with GFP?
Thanks!
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6 replies to this topic
#2
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Posted 01 September 2009 - 07:12 AM
PiDC, on Sep 1 2009, 10:44 AM, said:
Hi,
Does anyoone know if the anti-FLAG antibody M2 cross reacts with GFP?
Thanks!
Does anyoone know if the anti-FLAG antibody M2 cross reacts with GFP?
Thanks!
I never saw any cross reactivity with the Sigma F1804 M2 with turbo GFP, not sure about GFP or eGFP.
Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley
#3
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Posted 01 September 2009 - 07:31 AM
Dr Teeth, on Sep 1 2009, 05:12 PM, said:
PiDC, on Sep 1 2009, 10:44 AM, said:
Hi,
Does anyoone know if the anti-FLAG antibody M2 cross reacts with GFP?
Thanks!
Does anyoone know if the anti-FLAG antibody M2 cross reacts with GFP?
Thanks!
I never saw any cross reactivity with the Sigma F1804 M2 with turbo GFP, not sure about GFP or eGFP.
I don't know how different is turboGFP from eGFP... I am using eGFP, which comes from Aequorea victoria, and the M2 antibody gives a band, in my hands, that correspond to the size of GFP (in a lane loaded whith GFP).
Thanks for your help!
#4
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Posted 01 September 2009 - 08:18 AM
PiDC, on Sep 1 2009, 11:31 AM, said:
Dr Teeth, on Sep 1 2009, 05:12 PM, said:
PiDC, on Sep 1 2009, 10:44 AM, said:
Hi,
Does anyoone know if the anti-FLAG antibody M2 cross reacts with GFP?
Thanks!
Does anyoone know if the anti-FLAG antibody M2 cross reacts with GFP?
Thanks!
I never saw any cross reactivity with the Sigma F1804 M2 with turbo GFP, not sure about GFP or eGFP.
I don't know how different is turboGFP from eGFP... I am using eGFP, which comes from Aequorea victoria, and the M2 antibody gives a band, in my hands, that correspond to the size of GFP (in a lane loaded whith GFP).
Thanks for your help!
I suppose given the 8 amino acid epitope for FLAG, it is likely that it may cross react with many proteins.
FYI, eGFP is not from Aequorea victoria. It is a F64L point mutant of the wild-type GFP, which is from the jellyfish, resulting in an "enhanced" GFP that is brighter, and I believe also more yellow. Good luck with the Westerns!
Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley
#5
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Posted 01 September 2009 - 04:52 PM
Have you tried blasting the flag tag sequence against the gfp sequence?
#7
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Posted 25 September 2009 - 04:12 AM
Yes, I´ve seen it quite often. Flag antibody reacts with gfp and the other way around. If you have a big amount of Flag-protein your anti gfp antibody will recognize the flag-protein
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