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Mannitol in Lysis Buffer


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#1 JorgeP

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Posted 01 September 2009 - 05:00 AM

Hi!

Can anyone tell me why some Western Lysis Buffers use Mannitol? I need to make some westrns for ferritin and I found some protocols which use this reagent. I don't have it and I'm unsure whether I should buy it first or try the western without it. Thanks for your help.

Jorge

#2 genehunter

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Posted 01 September 2009 - 09:30 AM

I guess its for protein protection from denaturation. Protocols are made by people and a lot of protocols can be modified.




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