In my M.Sc. thesis work I found some E. coli with Ciprofloxacin resistance carrying plasmids of less than 6kb. After plasmid curing they became sensitive to ciprofloxacin. When I transformed DH5α with these plasmids they became resistant to cipofloxacin but I couldn't find any plasmid after plasmid extraction process. I carried out both calcium chloride method and electroporation techniques for transformation. For extraction process I used both Doly methad and commercial kit.
Please somebody give me some light.
Need help: Can't find plasmid in transformed cell!!
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