2 replies to this topic

[peritrophic]

I recovered DNA from gel by Millipore's kit,It's simple.Cut the band from gel and centrifuge for 10 minutes,but the efficiency is disappointed,only 20% or so. Can anyone reccomend me other methods?Thanks in advance

[Edwin]

I had initial problems with DNA extraction from gels. But I have no experience with the millipore kit. In our lab we always use the QIa EX kit by Qiagen. THeir recommendations are good enough. But I always get more than 75% when I use TE buffer (pH 8.0, which is very, very critical).
Guess you should give it a shot.

[bigbambu]

qiagen gel extraction kit works very well for me