Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How do I extract a HUGE plasmid?


  • Please log in to reply
1 reply to this topic

#1 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 01 February 2001 - 10:00 PM

I need to extract an 80-90 kb plasmid from E. coli. I can't find any protocols to accomlish this. Any ideas?

#2 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 02 February 2001 - 10:00 PM

What is the copy number of the plasmid? Why can't you use a Qiagen Kit? How much DNA do you require?

Best regards,

Andrew





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.