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How do I extract a HUGE plasmid?

Started by anonymous, Feb 01 2001 10:00 PM

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#1 anonymous

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Posted 01 February 2001 - 10:00 PM

I need to extract an 80-90 kb plasmid from E. coli.  I can't find any protocols to accomlish this.  Any ideas?

#2 anonymous

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Posted 02 February 2001 - 10:00 PM

What is the copy number of the plasmid? Why can't you use a Qiagen Kit?  How much DNA do you require?

Best regards,

Andrew

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