I am trying to download an alignment of Hepatitis B virus genotype C by searching with NCBI blast. I have an reference sequence of HBV geno C, but when I perform a blast search I also get a lot of sequences from other genotypes even animal HBV sequences. I have 2 questions here: first how can I use Entrez Query to limit my search (i.e. something like NOT genotype A, NOT genotype B etc.) because I cant seem to get the query syntax right! Second what is the best blast program of choice for this kind of search? I hope for a reaction!
Thanks in advance,
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