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Re: RNA quantitation

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#1 anonymous



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Posted 07 March 2001 - 10:00 PM

From: Nathalie Troffer
email: nathalie.troffer@inserm.u-strasbg.fr
Category: DNA-Related


I would like to find the best method for purifying a linearized plasmid from the fewcircular forms that always remain whatever purification method I use for the plasmidpreparation. I need a very pure result (to make a library) so purification on agarose isnot so good. Do you think sucrose gradient could be a method and do you have a protocol ?thanks for any response


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