Determining ideal page conditions
Posted 30 August 2009 - 10:05 AM
So I'm trying to seperate on a gel a 700kDa band from a 2000kDa band.
under my standard conditions (12% gel, 150v, 1-1.5hr) this obviously doesn't really happen.
I was wondering which conditions I should change and by about how much?
Posted 30 August 2009 - 04:13 PM
Posted 30 August 2009 - 04:38 PM
lower your gel percentage, adjust time/voltage as necessary.
Yeah I figured that, I was more hoping for a kind of idea how far to adjust.
i.e. is 8% gel sufficent or should I go lower?
should I run it for 2hr --> 3hr --> 4hr --> 6hr?
Posted 30 August 2009 - 06:26 PM
Posted 31 August 2009 - 07:34 AM
Gradient gels (eg. 4-10%) would be nice, if you can afford them...
Run until bromophenol blue is few mm from the bottom of gel.
For this molecular weight range size exclusion chromatography would be much better than PAGE.