ELISA Standard curves and sample concentration
Posted 30 August 2009 - 09:14 AM
Posted 30 August 2009 - 09:58 PM
Do you think i allow to check my Run 2 sample concentration using the Run 1 standard curve?
Itís tempting isnít it but afraid not, unless you have a good reason like the pipette tips fell off (Or possibly if you had run several plates and only one was out).
If you are mainly interested in comparing between samples rather than accurate quantitation the standard curve is not going to matter so much as long as the trends are the same for both plates.