I kept on having the problem of central cell seeding on two cancer cell lines when seeding the cells in 24 well plates. Before, seeding the cells, I tried to dislodge the cell pellet by flipping the falcon and pipette up and down several times. The cells were separated into individual cells when I did the cell counting by a haematocytometer. After seeding the cells, I tapped the plates gently/rocked the plate back and forth to make sure the cells were evenly distributed and waited for 5-10 mins to let the cells settle down. I observed the cells under a microscope and found most of the cells were distributed in the centre of the wells. Then, I repeated to do the tapping/rocking the plate again and put the plate aside and waited for 5-10 mins again. The same thing happened again!!! (P.S. my 24 well plates are flat-bottomed)
Are there some cell types having the tendency to move towards each other? Because this really affects my transfection experiment, what should I do to solve this problem?? Has anyone encountered this prolem???
Edited by Samantha, 30 August 2009 - 07:54 AM.