Hi
I've just started with the transfer of small RNA to nylon membrane. I do transfer at 10V using a semidry method for 1 hr, once the time exceeds for 15 mins and I saw the dye crossed the membrane to filter paper beneath , I wonder if the RNA can cross the membrane as well
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small RNA Northern
Started by Anand, Aug 30 2009 01:53 AM
1 reply to this topic
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Carlton H
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Posted 31 August 2009 - 05:04 PM
I wouldn't think that to be the case... How small is the RNA?
Or you could always just conclusively determine this yourself - rinse the filter paper and do RT-PCR.
Cheers,
-Carlton
Or you could always just conclusively determine this yourself - rinse the filter paper and do RT-PCR.
Cheers,
-Carlton
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