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Hello, please am working on random mutagenesis of bacillus cereus using pIC333. I stored my transformants on TSB containing both spectinomycin and erythromycin. After a mouth I pick single colonies restreaked them on same TSB containing antibiotics but there were no growth. Thus anyone has an idea what could have gone wrong. Is it that they loss the plasmid?
Feb 24 2013 06:54 PM
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