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Hello, I need help with western blot transfer process...I am looking for a low molecular weight protein (nearly 30 kDa)...I use invitrogen semi wet transfer system, the recommended voltage is 100 V for 1 hour, I am unable to reach that, my system adjusts it to 12 V even though the set V is 100, it never reaches that and hence get weak bands...is there any way to fix this problem?
Apr 29 2011 04:51 PM
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