Community Status Updates
uttess
Hello, I am trying to look for CHOP protein using SDS page...Is anyone working with this protein and could help me out with the transfer conditions as I get very weak bands using semi wet transfer at 18 V for 90 minutes on PVDF membrane...plz help!
Apr 30 2011 02:31 PM
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uttess
Hello, I need help with western blot transfer process...I am looking for a low molecular weight protein (nearly 30 kDa)...I use invitrogen semi wet transfer system, the recommended voltage is 100 V for 1 hour, I am unable to reach that, my system adjusts it to 12 V even though the set V is 100, it never reaches that and hence get weak bands...is there any way to fix this problem?
Apr 29 2011 04:51 PM
