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@ Shrubal	:	(19 December - 05:37 AM) I carried out a dot blot for various concentrations of my cell lysate and found weak positive staining for my protein of interest at a concentration of 15-25ng. However there was absolutely no staining at higher lysate concentrations (the positive control stained even at 100ng). Can someone tell me why? Thanks!
@ pito	:	(18 December - 06:48 AM) Clutz: ask this question on the forum! Make a post... The shoutbox is for short messages, not for questions like yours
@ clutz	:	(18 December - 04:36 AM) Hi everyone, In June 2012 I bought a number of antibodies from AbD Sertotec and, according to the data sheet from the STAR9B conjugate dated June 2012, the recommended working dilution should be between 1:200 and 1:500. but, in a later datasheet (from november 2013) the recommended working dilution is 1:25-1:100. My student asked the Serotec technical support and they told her to use the later STAR9B dilutions (2013) for our flow cytometry experiments: Bovine PBMC surface stained with purified Mo anti-Bov CD4+ Rabbit anti-mo IgG F(ab´)2-FITC and an intracellular staining with mo anti-bo IFNg-PE. We have got signals from IFNg in every sample and controls. We ran a test with a mo anti-bo CD4 FITC + mo anti-bo IFNgPE; and another test blocking with normal mo serum after de STAR9B staining and the stain with mo anti-bo IFNgPE, and the effect dissapeared, suggesting that the STAR9B is capturing the second conjugate as well... Is that possible??? Is it a matter of wrong dilution of STA9B conjugate and monovalent binding of STAR9B to de primary antibody? Please, help me to solve this mystery.... Thanks in advance
@ pito	:	(16 December - 01:19 PM) Hallo.
@ student47	:	(16 December - 07:27 AM) hi
@ Trof	:	(06 December - 07:51 AM) Nothing tells you more "have a nice weekend" like a spill of ml of beta-mercaptoethanol on the bench by a new colleague. I'm going to kill him as soon as my headache ends..
@ vetticus3	:	(29 November - 01:53 AM) different epitopes... all poly... all produce really beautiful IF... picking one and hoping for the best.
@ mdfenko	:	(28 November - 12:54 PM) they may be for different epitopes, poly and monoclonal, native and/or denatured protein (western blot or elisa/ip compatible
@ vetticus3	:	(28 November - 03:19 AM) Anyone have a good idea on how to pick a good antibody? The same comp has 3 for the same protein - getting confused. :S
@ jerryshelly1	:	(27 November - 02:33 PM) Everyone have a good Turkey Day!!
@ Inbox	:	(21 November - 12:11 PM) @Trof: nice. finishing 15th hrs non-desk work here.
@ Trof	:	(21 November - 12:47 AM) I just spend six hours writing. Need tea. And yes, it's 10 in the morning, here.
@ gfischer	:	(20 November - 07:27 AM) @Curtis And remember that a little stroking of the ego can never hurt
@ pcrman	:	(19 November - 06:30 PM) @Curtis just tell the other party what you intend to do, what you can offer, trying to get one interested and willing to work with you.
@ Curtis	:	(18 November - 09:31 PM) want to write a collaboration request. Not sure how to write it.
@ Curtis	:	(18 November - 08:08 AM) I never liked plate-based PCR machines.
@ Trof	:	(15 November - 02:16 PM) Whole new work experience.. unsticking optical PCR plate melted to the heated cover..
@ Curtis	:	(11 November - 12:32 AM) I'll make sure my zombies don't eat czech chiks
@ Trof	:	(10 November - 01:01 PM) @Curtis And the humankind is doomed!
@ Curtis	:	(06 November - 06:04 PM) Ph

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