http://www.protocol-online.org/forums Sun, 12 Feb 2012 08:09:01 +0000 1000 http://www.protocol-online.org/forums/topic/24386-alcohol-agglutination/ Hi!

1)Does alcohol cause blood cell agglutination?

Or agglutination in alcohol abuse possible in very high (lethal) promille dosage (50-100 promille)? Death - about 7 promille.

2)may alcohol change pH of blood ? may agglutination occur due to the changes pH of blood?

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ps/ i am use translator ]]> Sun, 12 Feb 2012 08:09:01 +0000 http://www.protocol-online.org/forums/topic/24386-alcohol-agglutination/ http://www.protocol-online.org/forums/topic/22645-isolating-plasma-from-frozen-blood-samples/
Question. I have frozen blood samples of mice. I need to isolate the blood plasma for protein detection. Does anyone know of a protocol to do this. First I thawed the whole blood sample, which is in EDTA coated vials. I tried spinning down at 3000 rpm for 10' but no clear plasma fraction was present. Also after spinning 14000rpm for 5' no clear fractionation.
Do I need to add something, or did the freezing to -80 already mess up my experiment.

hope you can help]]> Tue, 30 Aug 2011 14:17:42 +0000 http://www.protocol-online.org/forums/topic/22645-isolating-plasma-from-frozen-blood-samples/ http://www.protocol-online.org/forums/topic/19691-gamma-globulin/ why in arms-pcr we must change concentration between primers?
while there are their complementary sequence in template.]]> Tue, 22 Feb 2011 05:26:28 +0000 http://www.protocol-online.org/forums/topic/19691-gamma-globulin/ http://www.protocol-online.org/forums/topic/18940-hematopoietic-cells-separation/ Can anyone here suggest me the steps to separate those cells efficiently? -Thanks-

Regards,
Soosin]]> Tue, 11 Jan 2011 14:21:59 +0000 http://www.protocol-online.org/forums/topic/18940-hematopoietic-cells-separation/ http://www.protocol-online.org/forums/topic/18531-information-about-yemen-or-middle-east-technology-in-hospital/ Marco]]> Sun, 12 Dec 2010 18:17:53 +0000 http://www.protocol-online.org/forums/topic/18531-information-about-yemen-or-middle-east-technology-in-hospital/ http://www.protocol-online.org/forums/topic/18462-splitting-hemoglobin-into-monomers/ (split into monomers)? We have come up with KI-potassium iodide as the cheapest and
most easy to find means. It appears that dissociation into monomers minimizes hemolysis interference in some instances in a hemolyzed blood sample which arrives in a laboratory.
Any help is higly appreciated! Thank you in advance.]]> Wed, 08 Dec 2010 14:15:55 +0000 http://www.protocol-online.org/forums/topic/18462-splitting-hemoglobin-into-monomers/ http://www.protocol-online.org/forums/topic/18317-blood-storage/
I'm planning on using it as a base for a dried blood spot standard. Thanks.

--Grad]]> Wed, 01 Dec 2010 02:51:36 +0000 http://www.protocol-online.org/forums/topic/18317-blood-storage/ http://www.protocol-online.org/forums/topic/17736-how-to-make-percoll-solutions/
the percoll manual gives me the creeps really. i dont wanna study physics before being able to generate a percoll solution...

so is there anybody out there who could give me a "for dummies" procedure of how to generate this isotonic stock solution and then from that to get a 60% percoll solution?

many thanks!


bert


PS. i work with mouse bone marrow.]]> Tue, 26 Oct 2010 08:50:36 +0000 http://www.protocol-online.org/forums/topic/17736-how-to-make-percoll-solutions/ http://www.protocol-online.org/forums/topic/17643-blood-red-cells/ My lab has a collection of dried human blood (nucleated red blood cells) samples on Whatman cards. We are interested in extracting total proteins to be used in ELISA assay. Do you know if there is some protocol for this? I'd like to ask you if it's a good idea or not.
Thank you very much]]> Wed, 20 Oct 2010 10:07:04 +0000 http://www.protocol-online.org/forums/topic/17643-blood-red-cells/ http://www.protocol-online.org/forums/topic/17156-modified-pbs-buffer-for-red-blood-cells-washing/
A 1 liter 1X PBS solution consist of 8g of NaCl, 0.2g of KCl, 1.44g of Na2HPO4, 0.24g of KH2PO4

I plan to use it for washing my red blood cells.
However, I am lack of NaCl at the moment and have CaCl2 on hand.

If I were to replace NaCl with CaCl2,
should I go with equal weight (replace 8g of NaCl with 8g CaCl2), or equal molarity, in order to avoid hemolysis of my blood cells?

I'm unable to experiment it because I do not have spare blood samples.

Let me know how you think.

Thanks. ]]> Tue, 21 Sep 2010 17:57:23 +0000 http://www.protocol-online.org/forums/topic/17156-modified-pbs-buffer-for-red-blood-cells-washing/