http://www.protocol-online.org/forums/index.php?app=blog Community Blog List Syndication Fri, 09 Sep 2011 16:28:10 +0000 bbs@protocol-online.org (BioForum) IP.Blog 60 http://www.protocol-online.org/forums/blog/28/entry-38-tailoring-of-a-plaque-assay/


Today I will inspect the new HeLa that I awoke yesterday, proceed with infecting 3T3 cells (RE: a plaque assay) to gain the titer of four different aliquots of the mCMV strain, & turn in my progress report.

Excerpt from my report on the tailoring of a...]]> Fri, 09 Sep 2011 15:15:00 +0000 http://www.protocol-online.org/forums/blog/28/entry-38-tailoring-of-a-plaque-assay/ http://www.protocol-online.org/forums/blog/28/entry-37-plating-3t3-cells-for-a-plaque-assay/


I'd like to call...]]> Fri, 09 Sep 2011 05:13:00 +0000 http://www.protocol-online.org/forums/blog/28/entry-37-plating-3t3-cells-for-a-plaque-assay/ http://www.protocol-online.org/forums/blog/28/entry-36-just-some-pictures-i-think-look-cool/ ]]> Thu, 01 Sep 2011 16:06:00 +0000 http://www.protocol-online.org/forums/blog/28/entry-36-just-some-pictures-i-think-look-cool/ http://www.protocol-online.org/forums/blog/28/entry-35-interferon-protection-assay-day-3-inoculation/
Image: picture of my virus propagation

Yesterday (8/30/11) addition of 100ul PBL mIFN (interferon) {in a 5 fold serial dilution fashion} done in 1X DMEM + 10%FBS+PSG.
Today {8/31/11} plates (3) were subjected to 100uls of inoculum (EMCV in 1XDMEM+10%FBS+PSG)....]]> Wed, 31 Aug 2011 22:26:00 +0000 http://www.protocol-online.org/forums/blog/28/entry-35-interferon-protection-assay-day-3-inoculation/ http://www.protocol-online.org/forums/blog/28/entry-34-the-interferon-protection-assay/ Why:

The drug company our lab works for wants to test their antibodies conjugated to IFNs for antiviral activity. My task is to understand how much antibody-IFN is needed to "cure" an organism from a viral infection (specifically viruses which causes...]]> Tue, 30 Aug 2011 07:38:00 +0000 http://www.protocol-online.org/forums/blog/28/entry-34-the-interferon-protection-assay/ http://www.protocol-online.org/forums/blog/28/entry-31-tomorrow-a-new-day/ Fri, 12 Aug 2011 07:33:00 +0000 http://www.protocol-online.org/forums/blog/28/entry-31-tomorrow-a-new-day/ http://www.protocol-online.org/forums/blog/22/entry-30-thats-it/
Now I'm on my own, not funded, and I have to fork-out money to pay my semester fees.

The next semester starts coming August, but I do not have money even to pay my next month room rental.

I should have finished my project by now, however due to unavoidable...]]> Tue, 14 Jun 2011 14:38:00 +0000 http://www.protocol-online.org/forums/blog/22/entry-30-thats-it/ http://www.protocol-online.org/forums/blog/22/entry-29-my-wish/ Finally I got myself a new workstation for me to work on my papers and thesis, and of course I burnt all my savings which is around ~USD800.Just finished the first draft of my paper today, sent to both of my boss and hope they should stop angry with me. I still owe them another paper, since the patent of the primers was done and I should be able... Wed, 09 Feb 2011 10:56:00 +0000 http://www.protocol-online.org/forums/blog/22/entry-29-my-wish/ http://www.protocol-online.org/forums/blog/22/entry-26-is-time-for-a-new-workstation/ Fri, 17 Dec 2010 00:52:00 +0000 http://www.protocol-online.org/forums/blog/22/entry-26-is-time-for-a-new-workstation/ http://www.protocol-online.org/forums/blog/22/entry-25-all-i-got-was-frustrations/ Mon, 15 Nov 2010 04:38:00 +0000 http://www.protocol-online.org/forums/blog/22/entry-25-all-i-got-was-frustrations/