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Isolating keratinocytes for primary cell culture

09 Feb 2017

Hi, I was wondering if anyone has any experience in isolating keratinocytes from say patient...

  165 Views · 0 Replies


Strange 293T seeding

08 Feb 2017

Can anyone explain to me why my 293T cells are setting down in the pattern of the rack???  T...

  181 Views · 2 Replies ( Last reply by rkay447 )


How large need homology arms to be?

07 Feb 2017

Posted by Luca in Molecular Cloning
I would like to insert a 2808 sequence bihind a gene by CRISPR/Cas9 homology repair. Are 800 bp-h...

  135 Views · 1 Replies ( Last reply by bob1 )


IF Two Antigen Retrival Steps

31 Jan 2017

Posted by DavidEckert in Immunology
I have an interesting scenario. I'm focused on the colocalization of two proteins via IF. Protein...

  308 Views · 3 Replies ( Last reply by mdfenko )


Bisulfite conversion is complete except for the CpG sites...

25 May 2014

Hi all! Like many other scientists on this forum I'm having some issues with bisulfite conve...

  1,207 Views · 5 Replies ( Last reply by r03ml11 )

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