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patchy/holey bands after transfer, invasive bubbles?

03 Jul 2013

Dear BioForums 1st year post doc here. I literally did hundreds of westerns for my PhD. New setu...

  911 Views · 7 Replies ( Last reply by mdfenko )

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Use of urea in solubilizing protein and its aftermath

19 Jun 2016

Hi, so most of my protein that I wanted to express by E.coli BL21(DE3) is in inclusion bodies and...

  190 Views · 5 Replies ( Last reply by mdfenko )

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Weird results with bacterial transformation

16 May 2015

Posted by rmbio in Molecular Cloning
Hi all I have been doing cloning and transformation almost since past 10 years, but have nev...

  909 Views · 9 Replies ( Last reply by Andrea Fortina )

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Problem with subcloning genescript synthesized genes

23 Jul 2015

Posted by FoTem21 in Molecular Cloning
Hello! I received synthesized genes from Genescript in pUC57 vector two months ago. Since th...

  463 Views · 2 Replies ( Last reply by Andrea Fortina )

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Using SDS for DNA electrophoresis

07 Sep 2015

Posted by Raygoza in Molecular Cloning
Now, the question title may sound weird right off the bat, but my question is as follows: So...

  520 Views · 3 Replies ( Last reply by Andrea Fortina )


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