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Thank you... im still in the designing part of the experiment... so this really helps..^_^
We use BamHI from Invitrogen, routinely digest for just an hour in 30 - 50 ul volumes at 37C, and...
In principle, the order should make little difference. But, you can have all sorts of effects, su...
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Hi everybody,I got a problem in my western blots which is really annoying me.I dont know why I al...
Hi. Im a newbie here.. Does anyone know if the order of the genes in the plasmid have an effect o...
yeah, sequence it.To be 100% sure what it is, you need that specific strain with that genotype. i...
It's usually so that promoters don't read through and transripts don't produce overlapping sequen...