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You can use any sample you want as long as you have previous qpcr data for it.
I asked something similar in molecular but I think I should ask it here. I had 150ul of PCR...
Lower your gel percentage. I would use a 6% gel for this. Also check your running buffers, t...
My protein size is 260kDa and I am using 5% stacking gel and 7.5% resolving gel. After wet tank t...
I wish, no it seems they are taking it straight from the bottle and adding it to the bacterial br...
This is a small lab outshoot, we're lucky to even have the spectrophotometer. After the PCR...
You wrote "...i ran the DNA on 0.8% agarose without EtBr...". Do you mean you forgot the staining...
If your sticky end ligation is going to work, it will work with a 10 minute room temperature liga...