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Problems with SgrAI digest, ligation, killer cut and weir...

15 Dec 2014

Hello BioForum community, This is my first post here so apologies if it’s not perfect. I h...

  175 Views · 4 Replies ( Last reply by Sharknami )


Detection of Td-Tomato protein in frozen sections

09 Dec 2014

Posted by Anana in Cell Biology
Hello,We are working with  Wnt1-cre:Rosatomato mice and have trouble detecting red...

  114 Views · 2 Replies ( Last reply by Rsm )


Inconsistent colony PCR result

15 Dec 2014

Hi,  I am doing bac to bac transformation of DH10Bac. Blue white screening was used to...

  123 Views · 2 Replies ( Last reply by Lian )


Problem with BamHI Digestion

14 Dec 2014

Hello,    I am a student in a Biotechnology program and as part of my Mol Bio clas...

  161 Views · 6 Replies ( Last reply by Jeannine Daniels )


Plasmid Methylation

14 Dec 2014

Posted by Raygoza in Molecular Cloning
Hi, does anyone know if there exist a commercial enzyme or protocol to remove methylation of dna...

  95 Views · 3 Replies ( Last reply by bob1 )

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