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Reducing contamination in 16s PCR for metagenomic library...

14 Apr 2015

Hi everybody, Recently i came across with the contamination with he PCR kits while i was trying...

  140 Views · 4 Replies ( Last reply by roshanbernard )


Efficient lysis of E.coli cells

06 Apr 2015

Posted by neuron in Microbiology
Hi, We are trying to lyse e.coli cells wherein we don't want to use sonicator and we don't h...

  145 Views · 4 Replies ( Last reply by phage434 )


How do I know the protein structure?

16 Apr 2015

Hi guys,  I am trying to construct a fusion protein and wish to avoid one protein being...

  85 Views · 3 Replies ( Last reply by phage434 )


PCR anomaly in a mid-range dilution of the template

15 Apr 2015

Hi guys, I'm trying to amplify cDNA using amplified cDNA as template (amplified using GeneRa...

  121 Views · 1 Replies ( Last reply by phage434 )


Cloning of an unknown gene

13 Apr 2015

Posted by bio_kid in Molecular Cloning
Hi ! I would appreciate some help on my assingment, I have an idea but I would like to hear...

  123 Views · 3 Replies ( Last reply by phage434 )

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