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Degenerate primers

Yesterday, 08:33 AM

Hi I have to sets of primers, the first set is suppose to amplify 242 bp product and th...

  87 Views · 3 Replies ( Last reply by Trof )


how to draw a correlation plot between IC50 values and pr...

Yesterday, 12:46 PM

Hi all, Help needed here with stats... I am trying to do a correlation between IC50 val...

  70 Views · 0 Replies


RNA gel problem (melted bands)

Yesterday, 08:15 AM

Dear all, I extracted RNA from Chlamydomonas cells with the traditional phenol:CHCl3 protoco...

  86 Views · 1 Replies ( Last reply by phage434 )


Low Yield Miniprep

20 Jan 2015

Posted by cytheon in Molecular Cloning
Hi everyone, I'm having a problem with my miniprep yields. Usually I get 100-300 ng/uL with...

  221 Views · 5 Replies ( Last reply by labtastic )


How do I extract soluble protein after adding SDS-PAGE bu...

26 Jan 2015

Hi everyone, I had a bit of a mess up in the lab today. I'm currently trying to overexpress...

  202 Views · 1 Replies ( Last reply by labtastic )

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