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How to check if my primers were degraded?

11 Aug 2017

Hello!I try to reproduce an experiment that I did abroad some years ago. It was a preliminar...

  159 Views · 1 Replies ( Last reply by labtastic )


calculating ramp rate on thermocycler - urgent

12 Aug 2017

My thermocycler has a ramp rate of 5C/sec. I have a PCR program that needs to go from 55*C to 72*...

  150 Views · 1 Replies ( Last reply by bob1 )


Agarose beads IP troubles

10 Aug 2017

I'm new in proteomics and doing IP with A/G agarose beads, but I struggle with what is the best w...

  193 Views · 6 Replies ( Last reply by mdfenko )


Funny shaped PCR curve

31 Jul 2017

Ive been using a custom made mastermix that for some reason results in a qPCR curve that has a ba...

  308 Views · 1 Replies ( Last reply by bob1 )


FAC Sorting of live cells based on intracellular protein...

06 Aug 2017

Posted by neuropath in Flow Cytometry
Hi Bioforumers, Has anyone successfully FAC sorted live cells based on their expression of a...

  309 Views · 0 Replies

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