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"Leaky" stepwise elution of active protein from I...

01 Apr 2016

Hello, I am working on purification of a cytotoxin from spent growth media (cell free supern...

  280 Views · 2 Replies ( Last reply by Chris22 )


Formula to calculate bacterial doubling time

18 Apr 2016

Posted by Meg P. Anula in Microbiology
Hi all, I've checked the OD600 of my bacteria culture every hour and derive the doubling tim...

  262 Views · 3 Replies ( Last reply by Meg P. Anula )


Increasing protein solubility in general

15 Apr 2016

I am looking for general techniques/additives to buffer to increase protein solubility and reduce...

  216 Views · 3 Replies ( Last reply by Missle )


Best protocol to solubilize bacterial fusion proteins?

08 Apr 2016

Hello everyone I need your advice for the solubilisation of a His tagged protein produced in E.co...

  193 Views · 5 Replies ( Last reply by mdfenko )


Wrong or No fragment in Directional Cloning Vector

24 Mar 2016

Dear helpful community, I am having trouble PCR-amplifying and cloning a ~2.5kb fragment int...

  677 Views · 8 Replies ( Last reply by aodonnell311 )

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