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DNA precipictation at -80 went thick

20 Dec 2017

Hi all, I've just done normal DNA precipitation in a 50ml falcon (DNA in 10ml H20, 0.1 volum...

  289 Views · 2 Replies ( Last reply by Trof )

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SDS precipitation in cold

09 Jan 2018

Hi everyone, Does anyone know how to prevent 2% SDS from precipitating in cold (4*C)? Can I...

  156 Views · 1 Replies ( Last reply by Trof )

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Viscous plasmids and transfection issues

09 Jan 2018

Posted by Trof in Molecular Biology
I have a question, that feel way to basic, but since I changed the workplace, things are done dif...

  146 Views · 1 Replies ( Last reply by merlav )

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Immunoprecipitation (IP) washes

15 Dec 2017

Posted by DrSnood in Molecular Biology
Hi all, Lab debate: Do you need to rotate your samples during the wash steps for an IP? Ther...

  195 Views · 2 Replies ( Last reply by Trof )

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Directional RNAseq Library Prep for NGS. How does it work?

05 Jan 2018

Posted by liguerr in Molecular Biology
Recently we have been looking at mRNA transcript patterns in RNA viruses using NGS. I am currentl...

  211 Views · 1 Replies ( Last reply by Trof )


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