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Thrombin cleaved GST-fusion protein- multiple bands

Yesterday, 07:41 AM

Hi guys,I am trying to purify my protein of interest of about 72KDa from GST-fusion tag using thr...

  49 Views · 1 Replies ( Last reply by labtastic )


standards for qPCR and some other doubts

18 May 2015

Hello, 1) I want to know how can i prepare standards for my qPCR. I will extract RNA from th...

  120 Views · 8 Replies ( Last reply by Mad Researcher )


control shRNA

19 May 2015

Posted by Lee6948 in Cell Biology
Hi In my last experiment, I tried to determine which shRNA contruct of CXCL2 gene should me...

  89 Views · 2 Replies ( Last reply by Lee6948 )


MDA-kb2 cells - problem with luciferase expression

17 May 2015

Dear all, I am working with MDA-kb2 cells which are supposed to be used for agonist/antagoni...

  118 Views · 4 Replies ( Last reply by Andrzej Chalupniak )


Primer dilution

20 May 2015

Hello, I received my primers today (in a plate) and the datasheet specified to add 100 ul of...

  102 Views · 7 Replies ( Last reply by phage434 )

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