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how to do % input calculations from Ct values

19 Jul 2017

Hi All, I am currently standardizing the ChiP protocol in my lab and very naive in the techn...

  98 Views · 0 Replies


large scale protein purification

03 Sep 2015

Hi all, can somebody suggest bulk scale protein purification protocols for proteins produced extr...

  786 Views · 6 Replies ( Last reply by paramyosin )


Tagged recombinant protein has different mobility?

28 Nov 2012

I did an expression of Adh gene in BL21 (DE3). I purified the protein using histidine tag column....

  1,951 Views · 7 Replies ( Last reply by paramyosin )


Couldn't detect Vimentin in western blot, in spite of...

17 Jul 2017

I work on Epithelial to Mesenchymal Transition (EMT) using MDA-MB-468 as a model cell line. I use...

  142 Views · 0 Replies


We need a way to see microRNA and DNA in vitro for revers...

16 Apr 2017

Imagine being able to observe microRNA and DNA action in vitro, that, with the help of computer v...

  598 Views · 8 Replies ( Last reply by Fredreload )

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