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Can mutations be created during cloning?

12 Sep 2014

Posted by Arrate in Molecular Cloning
Hello, I have cloned my PCR product using the TOPO-TA cloning kit (Life Technologies) and th...

  146 Views · 4 Replies ( Last reply by bob1 )


Moving "rods" in human PBMC culture?

02 Dec 2011

Hello everybody in our lab we have to isolate PBMCs from human blood using Ficoll and culture t...

  1,490 Views · 7 Replies ( Last reply by bob1 )


Methods to Sequencing "Unknown" DNA

Yesterday, 09:25 AM

Posted by djvan in Molecular Biology
Hello, I'm trying to characterize a naturally occurring deletion in an animal model.  T...

  100 Views · 2 Replies ( Last reply by djvan )


sequence assembly program

Today, 02:20 AM

Hallo, anyone here that knows a good (free) program to assemble some sequences?I am using a...

  54 Views · 4 Replies ( Last reply by pito )


protein biotinylation

Today, 04:39 AM

Hi everybody,I have some problem with the biotinylation protocol for a protein and I need your he...

  30 Views · 0 Replies

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