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reason for curved bands?

Today, 07:53 AM

I have noticed this with highly concentrated samples - loaded 3-4ul of sample with same volume of...

  9 Views · 0 Replies


Clean ligation controls but no insert

Yesterday, 10:23 PM

Posted by miST32 in Molecular Cloning
Hi all, I've been working to clone several mutant alleles of the same gene into a new expression...

  63 Views · 2 Replies ( Last reply by miST32 )


How to avoid false positive results due to the presence o...

Today, 04:58 AM

Dear sirs Kindly give a guidelines/advice how can we avoid false positive results due to the...

  29 Views · 0 Replies


Can't detect GAPDH (western) with Alexa488 secondary...

21 Oct 2014

I ran 10ug total protein (from RIPA harvest) my lanes and used Kaleidoscope protein standard....

  156 Views · 6 Replies ( Last reply by mdfenko )


Agarose gel electrophoresis uneven migration

20 Oct 2014

Posted by sayk in Molecular Biology
I  use 1.3% agarose for my restriction digestion, it comes out wavy and irregular this start...

  236 Views · 8 Replies ( Last reply by mdfenko )

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