- Biotinylation (Elham Mollazade, Yesterday, 10:18 PM)
- Would IP make the protein of interest run slower on SDS-PAGE? (DrZeus, Yesterday, 06:29 PM)
- cell seeded 3D collagen 1 gel (GMARATHA, Yesterday, 04:04 AM)
- anyone has experience in adhesion assay? (gyma, May 20 2013 11:19 PM)
- ChIP, Sonication and SDS (j47027, May 20 2013 03:37 PM)
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110 Views · 1 Replies ( Last reply by pcrman )
I am looking for some means to have a hint about the targets of some unknown micro RNAs. Are there any bioinformatic tools that can predict the target of an unknown micro RNA? The micro RNA sequences in my hand are not related to known classes of miRNAs. My experimental organism is Drosophila. Any suggestions will be greatly appreciated
1,008 Views · 2 Replies ( Last reply by pcrman )
also the gene location of GHR of water buffalo with refrences.
pleases can anybody help me out?
53 Views · 1 Replies ( Last reply by pcrman )
50 Views · 1 Replies ( Last reply by pcrman )
Clone 1: sequence at 5' end was the reverse complement of the 3' end - i.e. my reverse primer
Clone 2: sequence at 5' end was forward primer and 3' end was reverse primer. Seems correct, but blast results show sequence is similar to some mouse cloned product.
Clone 3: sequence at 5' end was the reverse complement of the 3' end - i.e. my reverse primer
The sequences matches the primers are 100% correct but not sure what the sequence is between the primers. The sequencing peaks look clean, sharp and good height. All sequence are the same size. Clone 1 is about 98% similar to clone 3 but both have no similarity to Clone 2. My non-template control does not produce anything.
No idea where to start, except to start over?
Sample problem - mRNA contamination?
Primer issues? Primers are reported in the literature.
Cloning or sequencing issue?
54 Views · 1 Replies ( Last reply by pcrman )
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Would IP make the protein of interest run slower on SDS-PAGE?
DrZeus - Yesterday, 06:29 PM
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What specifically is your problem or question?
Bioinformatic tools for novel miRNA target prediction
pcrman - Yesterday, 11:29 PM
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