Latest Discussions

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  Should Lineage marker cocktail be used in cell lines to remove lineage specific

  rv1234 - Yesterday, 10:07 PM

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  Optimization of real time-PCR - where should I start from?

  Nephrite - Apr 17 2018 09:11 PM

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  Pyura DNA extraction

  Andr?s_inzunza - Apr 17 2018 11:48 AM

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  Favorite polymerase for general cloning?

  DrSnood - Apr 17 2018 11:28 AM

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  Calculation help

  Mad Researcher - Apr 17 2018 10:39 AM

Recently Added Posts

• 
  Should Lineage marker cocktail be used in cell lines to remove lineage specific

  rv1234 - Yesterday, 10:07 PM

  Hi, I am isolating some specific sub-populations from breast cancer cell lines. The biomarke...

• 
  Optimization of real time-PCR - where should I start from?

  bob1 - Yesterday, 06:18 AM

  1) As you specified relative quantitation you don't need to do standard curves; those would be fo...

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  Pyura DNA extraction

  El Crazy Xabi - Apr 17 2018 11:19 PM

  That looks like a usual gDNA extraction. A bit degraded, but the samples next to the ladder seem...

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  Optimization of real time-PCR - where should I start from?

  Nephrite - Apr 17 2018 09:11 PM

  Hello!Here I have 36-well Rotor gene 6000 cycler and I want to do relative quantitation via two s...

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  restriction enzyme pcr cloning

  bob1 - Apr 17 2018 12:54 PM

  It should be fine. Note that blunt end cloning is orders of magnitude less efficient than sticky...

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  Severe or entire Blur formation in my SDS PAGE.

  aimikins - Apr 17 2018 12:53 PM

  can you post a picture? often distortion can be due to excessive glycerol in the buffer, but ther...

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  Calculation help

  bob1 - Apr 17 2018 12:49 PM

  See attachment to post here: http://www.protocol-online.org/forums/topic/5838-cell-counting/...

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  Favorite polymerase for general cloning?

  bob1 - Apr 17 2018 12:46 PM

  I've used KOD in the past. Plenty of people use Pfu

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