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Thanks a lot Bob1!
thanks for the reassurance the transfer was ok.Added the SDS and it looks better now, too. T...
I incubated the transformed DH5alpha for 1hr at 37 in SOC, and the plasmid is Amp resistant
what did you do? what are we looking at (don't say "an agarose gel")?
It's worth a try. Did you do an outgrowth following transformation?
Are you looking at the called sequence, or at the electropherogram. Mis-called bases are common a...
i'm not sure i understand what it is that you are doing. pcr will amplify dna, sds-page is for th...
Hey, I transformed some cells on Tuesday, and plated onto the wrong antibiotic plate. I kept the...