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DNA/RNA extraction from sputum

09 Feb 2010

Posted by kfszelok in Microbiology
Is there any standard protocol for DNA/RNA extraction for sputum which is viscous? I am am going...

  1,066 Views · 3 Replies ( Last reply by Chris22 )

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How to select promoter for resistance gene in linear/nake...

Yesterday, 10:43 AM

Posted by Chris22 in Microbiology
Hello, Short version:How do you pick a constitutively expressed promoter from a bacterial ge...

  46 Views · 2 Replies ( Last reply by Chris22 )

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How to clone a 2A peptide between two ORFs?

29 Mar 2015

Posted by 5280 in Molecular Cloning
I want clone two ORFs within a single vector using the 2A peptide instead of the IRES. I have a f...

  71 Views · 1 Replies ( Last reply by Robin Shen )

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Cross-species genes on the chromosomes

23 Mar 2015

Dear all,  While searching for a cross-species conservation of miRNAs genes (in terms o...

  141 Views · 4 Replies ( Last reply by stéphaniefath )

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Beads pull down only free GST

Today, 01:48 AM

Hey you all, i am currently trying to express a protein in a GST-vector (pGEX6p1). I already...

  33 Views · 8 Replies ( Last reply by Franzi )


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