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General Lab Techniques 4 articles

DNA migrate differently in agarose vs PAGE gel
Aug 15 2011  Many things can affect DNA migration in gel. Check this page "Top 10 Fun Facts for DNA Electrophoresis" to see if there is anything that might have caused the abnormal migration....

PCR 3 articles

How to get rid of PCR primer dimer
Aug 02 2014  1) Try the DMSO up to 5%. Some polymerases are ok with DMSO and others aren't. You never know until you try. 2) Try a two step PCR. There is a paper in Biotechniques about a two...

Western Blotting 0 articles

There are no articles in this category yet.

RNA Methods 2 articles

Handling RNA - How to avoid RNase contamination?
--  Hi Susan, I would second what tea-test has pointed out. Any tissue you process is going to be loaded with RNases (cells contain RNases). Any residual RNase present on your homogenizer...

Molecular Cloning 0 articles

There are no articles in this category yet.

Cell Culture 2 articles

How to coat coverslips before seeding cells?
Aug 22 2011  jennycharlies Posted 18 August 2011 - 06:55 AM Hi, I am going to seed my cells onto coverslips inside the wells of a 12 well plate and was wondering what is the best way to coat the...

Stem Cells 0 articles

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General Molecular Biology Methods 0 articles

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Transcription, epigenetic, epigenomic techniques 4 articles

Sonication Vs MNase for ChIP assay
Jul 28 2014  I am sure that this debate has been covered before, but I just wanted to share my own personal experiences with it. I am working with a TF and fairly recently switched to MNase. I initial...

Immunology 1 articles

Subcategories
Issues Might Meet in the Process of Using ELISA...
Jul 03 2013  In order to help improve the precision of an assay, Creative Biomart experts concluded the following tips for reference: Issues Reasons Tips...

RNAi, miRNA and ncRNA 0 articles

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Bioinformatics and Biostatistics 1 articles

How to find promoter sequence for methylation s...
Mar 24 2014  Many people have problem identifying or predicting the promoter sequence of a gene, or don't know how to get the actual sequence for analysis such as primer design, transcription fact...

Protein Methods 0 articles

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FACS Analysis 0 articles

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