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DNA migrate differently in agarose vs PAGE gel

Aug 15 2011 08:59 PM | pcrman in Electrophoresis

i am currently working a gene in leukemia. The mutant allele has been sequenced to contain 527 base pair. when i run an agarose gel to confirm, it did appear in the position approximately equal to 527 base pair according to the DNA ladder. HOWEVER, whe...

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Tips on freezing and thawing of cells

Sep 10 2004 12:32 PM | bioforum in Cell Culture

I am facing problems in thawing of cells. The procedure which we follow is- we take stock from liq. N2 then though it in water bath at 37 degree. Then we take medium in falcon (5ml) then add thawed cells dropwise into it. We then centrifuge it at 1500...

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Normalization for RNA or cDNA during two step R...

Apr 20 2009 09:13 AM | bioforum in General Lab Techniques

Curtis 20 April 2009 - 03:13 AM Hello all, I have a two-step SYBR Green RT-PCR kit for RotorGene. After I extract RNA from my samples, I proceed with cDNA synthesis right away without measuring the RNA concentration or wit...

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How to avoid foaming during sonication in ChIP...

Feb 01 2009 01:33 PM | bioforum in ChIP Assay

ChIP - Foaming in sonication and other woes - <small> (Jan/27/2005 )</small>Hi all! I was wondering if anyone out there has got any trick to avoid foaming during sonication in the SDS lysis buffer for ChIP assays. Supossedly, placi...

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Useful Dilution Techniques & Calculations

Mar 21 2009 02:33 PM | bioforum in General Lab Techniques

Today was a very bad day at the university... beside that some students answered in the quiz question that if you get blue color in Gram staining that means your bacteria is Gram negative :huh: I noticed most of the students including myself had troub...

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How to amplify long fragment from bisulfite tre...

I am able to very successfully get small (150-200 bp) products using a nested PCR strategy on bisulfite modified DNA; I do not see products from the larger primers but they are certainly there since the nest works so well. I'd love if I could sequ...

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Differences between MSP and BSP methods

Hello everyone I'm new here and I was wondering if I could get some assistance. In my research of the literature I thought the differences between the two methods were based on the proximity of the primers to the CpG sites and that BSP primers...

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