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- General Lab Techniques
- Western Blotting
- RNA Methods
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- Transcription, epigenetic, epigenomic techniques
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- Bioinformatics and Biostatistics
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Transcription, epigenetic, epigenomic techniques Archives
ChIP - Foaming in sonication and other woes - <small> (Jan/27/2005 )</small>Hi all! I was wondering if anyone out there has got any trick to avoid foaming during sonication in the SDS lysis buffer for ChIP assays. Supossedly, placi...
Hello everyone I'm new here and I was wondering if I could get some assistance. In my research of the literature I thought the differences between the two methods were based on the proximity of the primers to the CpG sites and that BSP primers...
I am able to very successfully get small (150-200 bp) products using a nested PCR strategy on bisulfite modified DNA; I do not see products from the larger primers but they are certainly there since the nest works so well. I'd love if I could sequ...
I am sure that this debate has been covered before, but I just wanted to share my own personal experiences with it. I am working with a TF and fairly recently switched to MNase. I initially tried MNase digestion out of pure curiousity, but I have to sa...