Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

(View All Videos) Toggle BioVideo


Transcription, epigenetic, epigenomic techniques Archives

How to avoid foaming during sonication in ChIP...

Feb 01 2009 01:33 PM | bioforum in ChIP Assay

ChIP - Foaming in sonication and other woes - <small> (Jan/27/2005 )</small>Hi all! I was wondering if anyone out there has got any trick to avoid foaming during sonication in the SDS lysis buffer for ChIP assays. Supossedly, placi...

Read story →    0 comments    -----

Differences between MSP and BSP methods


Hello everyone I'm new here and I was wondering if I could get some assistance. In my research of the literature I thought the differences between the two methods were based on the proximity of the primers to the CpG sites and that BSP primers...

Read story →    0 comments    -----

How to amplify long fragment from bisulfite tre...


I am able to very successfully get small (150-200 bp) products using a nested PCR strategy on bisulfite modified DNA; I do not see products from the larger primers but they are certainly there since the nest works so well. I'd love if I could sequ...

Read story →    0 comments    -----

Sonication Vs MNase for ChIP assay

Apr 14 2011 09:59 AM | Dukey in ChIP Assay

I am sure that this debate has been covered before, but I just wanted to share my own personal experiences with it. I am working with a TF and fairly recently switched to MNase. I initially tried MNase digestion out of pure curiousity, but I have to sa...

Read story →    2 comments    -----

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.