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How to avoid contamination...
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Transcription, epigenetic, epigenomic techniques Archives
Sonication Vs MNase for ChIP assay
Apr 14 2011 09:59 AM | Dukey in Transcription, epigenetic, epigenomic techniques
I am sure that this debate has been covered before, but I just wanted to share my own personal experiences with it. I am working with a TF and fairly recently switched to MNase. I initially tried MNase digestion out of pure curiousity, but I have to sa...
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How to avoid foaming during sonication in ChIP...
Feb 01 2009 01:33 PM | bioforum in Transcription, epigenetic, epigenomic techniques
ChIP - Foaming in sonication and other woes - <small> (Jan/27/2005 )</small>Hi all! I was wondering if anyone out there has got any trick to avoid foaming during sonication in the SDS lysis buffer for ChIP assays. Supossedly, placi...
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How to amplify long fragment from bisulfite tre...
Jan 27 2005 07:15 PM | pcrman in Transcription, epigenetic, epigenomic techniques
QuoteI am able to very successfully get small (150-200 bp) products using a nested PCR strategy on bisulfite modified DNA; I do not see products from the larger primers but they are certainly there since the nest works so well. I'd love if I could...
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Differences between MSP and BSP methods
Mar 02 2005 12:16 AM | bioforum in Transcription, epigenetic, epigenomic techniques
QuoteHello everyone I'm new here and I was wondering if I could get some assistance. In my research of the literature I thought the differences between the two methods were based on the proximity of the primers to the CpG sites and that BSP primer...
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