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How to avoid foaming during...
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How to find promoter sequen...
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Issues Might Meet in the Pr...
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Can 18s gene be used as ref...
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RT-PCR
Can 18s gene be used as reference gene for RT-PCR?
Hi, I have done few real time PCR works recently, and I used the 18s gene for endogeneous control. Its Ct value was approximately 11 for every cDNAs used as template. Here, I have a question. Since 18s gene doesn't have poly A tail, it can't...
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real time PCR, house keeping gene and 2 more...
How to get rid of PCR primer dimer
1) Try the DMSO up to 5%. Some polymerases are ok with DMSO and others aren't. You never know until you try. 2) Try a two step PCR. There is a paper in Biotechniques about a two step PCR reaction but I don't have the reference with me sin...
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