Submit your paper to J Biol Methods today!
Categories See All
Search Articles
Recent Articles
Recent Comments
-
Issues Might Meet in the Pr...
By Ashlay Donlad, Jun 30 2016 05:39 AM -
Can 18s gene be used as ref...
By Ashlay Donlad, Mar 28 2016 06:47 AM -
How to find promoter sequen...
By Ashlay Donlad, Feb 05 2016 05:57 AM -
Sonication Vs MNase for ChI...
By Ashlay Donlad, Feb 05 2016 05:13 AM -
How to get rid of PCR prim...
By Anh Nguyen, Aug 02 2014 05:48 AM -
Sonication Vs MNase for ChI...
By Boukaba, Jul 28 2014 11:17 PM
1
RT-PCR
Can 18s gene be used as reference gene for RT-PCR?
Hi, I have done few real time PCR works recently, and I used the 18s gene for endogeneous control. Its Ct value was approximately 11 for every cDNAs used as template. Here, I have a question. Since 18s gene doesn't have poly A tail, it can't...
Read story → 1 comments
real time PCR, house keeping gene and 2 more...
How to get rid of PCR primer dimer
1) Try the DMSO up to 5%. Some polymerases are ok with DMSO and others aren't. You never know until you try. 2) Try a two step PCR. There is a paper in Biotechniques about a two step PCR reaction but I don't have the reference with me sin...
Read story → 2 comments
