Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

(View All Videos) Toggle BioVideo

Worm Picking

(4,256 Views)

07 Mar 2013
PCR Cloning

(4,439 Views)

24 Jul 2013
Using NCBI for RT-PCR Prime...

(6,410 Views)

23 May 2013
Aseptic Technique - Part 3

(4,413 Views)

05 Mar 2013
Prepare hESCs for Cryoprese...

(5,026 Views)

24 Jul 2013
siRNA transfection procedures

(4,314 Views)

23 May 2013

Search Articles

Recent Articles

Recent Comments

How to avoid foaming during...
By michaelddorsey, Feb 18 2019 05:49 AM
How to find promoter sequen...
By Kikogarcia, Nov 15 2018 01:29 AM
Issues Might Meet in the Pr...
By Ashlay Donlad, Jun 30 2016 05:39 AM
Can 18s gene be used as ref...
By Ashlay Donlad, Mar 28 2016 06:47 AM
How to find promoter sequen...
By Ashlay Donlad, Feb 05 2016 05:57 AM
Sonication Vs MNase for ChI...
By Ashlay Donlad, Feb 05 2016 05:13 AM

1

RT-PCR

Can 18s gene be used as reference gene for RT-PCR?

Mar 10 2013 06:31 PM | BMF in RT-PCR

Hi, I have done few real time PCR works recently, and I used the 18s gene for endogeneous control. Its Ct value was approximately 11 for every cDNAs used as template. Here, I have a question. Since 18s gene doesn't have poly A tail, it can't...

Read story → 1 comments

real time PCR, house keeping gene and 2 more...

How to get rid of PCR primer dimer

Jan 29 2005 08:06 PM | bioforum in RT-PCR

1) Try the DMSO up to 5%. Some polymerases are ok with DMSO and others aren't. You never know until you try. 2) Try a two step PCR. There is a paper in Biotechniques about a two step PCR reaction but I don't have the reference with me sin...

Read story → 2 comments

Google Sign in options