Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

(View All Videos) Toggle BioVideo

DNA Barcoding Protocol: Iso...

(2,165 Views)

30 Jul 2013
Aseptic Technique - Part 1

(2,143 Views)

05 Mar 2013
Arabidopsis Seed Planting P...

(1,996 Views)

30 Jul 2013
Transfection, Part I - Prep...

(2,255 Views)

13 Mar 2013
How to Measure PCR Efficien...

(2,512 Views)

12 Mar 2013
siRNA transfection procedures

(2,230 Views)

23 May 2013

Search Articles

Recent Articles

Recent Comments

Issues Might Meet in the Pr...
By Ashlay Donlad, Jun 30 2016 05:39 AM
Can 18s gene be used as ref...
By Ashlay Donlad, Mar 28 2016 06:47 AM
How to find promoter sequen...
By Ashlay Donlad, Feb 05 2016 05:57 AM
Sonication Vs MNase for ChI...
By Ashlay Donlad, Feb 05 2016 05:13 AM
How to get rid of PCR prim...
By Anh Nguyen, Aug 02 2014 05:48 AM
Sonication Vs MNase for ChI...
By Boukaba, Jul 28 2014 11:17 PM

1

RT-PCR

Can 18s gene be used as reference gene for RT-PCR?

Mar 10 2013 06:31 PM | BMF in RT-PCR

Hi, I have done few real time PCR works recently, and I used the 18s gene for endogeneous control. Its Ct value was approximately 11 for every cDNAs used as template. Here, I have a question. Since 18s gene doesn't have poly A tail, it can't...

Read story → 1 comments

real time PCR, house keeping gene and 2 more...

How to get rid of PCR primer dimer

Jan 29 2005 08:06 PM | bioforum in RT-PCR

1) Try the DMSO up to 5%. Some polymerases are ok with DMSO and others aren't. You never know until you try. 2) Try a two step PCR. There is a paper in Biotechniques about a two step PCR reaction but I don't have the reference with me sin...

Read story → 2 comments

Google Sign in options