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- General Lab Techniques
- Western Blotting
- RNA Methods
- Molecular Cloning
- Cell Culture
- Stem Cells
- General Molecular Biology Methods
- Transcription, epigenetic, epigenomic techniques
- RNAi, miRNA and ncRNA
- Bioinformatics and Biostatistics
- Protein Methods
- FACS Analysis
PCR contaminations are like ghosts... you know they are there but you dont know WHERE?? I have followed the following with good results... Prepare your master mix in a separate room from your current location, somewhere PCR is not the main practice....
1) Try the DMSO up to 5%. Some polymerases are ok with DMSO and others aren't. You never know until you try. 2) Try a two step PCR. There is a paper in Biotechniques about a two step PCR reaction but I don't have the reference with me sin...
QuoteHi, I have done few real time PCR works recently, and I used the 18s gene for endogeneous control. Its Ct value was approximately 11 for every cDNAs used as template. Here, I have a question. Since 18s gene doesn't have poly A tail, it can...