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Pinned  How to avoid contamination in PCR?

Oct 02 2004 12:24 PM | bioforum in PCR

PCR contaminations are like ghosts... you know they are there but you dont know WHERE?? I have followed the following with good results... Prepare your master mix in a separate room from your current location, somewhere PCR is not the main practice....

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How to get rid of PCR primer dimer

Jan 29 2005 08:06 PM | bioforum in RT-PCR

1) Try the DMSO up to 5%. Some polymerases are ok with DMSO and others aren't. You never know until you try. 2) Try a two step PCR. There is a paper in Biotechniques about a two step PCR reaction but I don't have the reference with me sin...

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Can 18s gene be used as reference gene for RT-PCR?

Mar 10 2013 06:31 PM | BMF in RT-PCR

Hi, I have done few real time PCR works recently, and I used the 18s gene for endogeneous control. Its Ct value was approximately 11 for every cDNAs used as template. Here, I have a question. Since 18s gene doesn't have poly A tail, it can't...

Read story →    2 comments    -----    real time PCR, house keeping gene and 2 more...

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